MSCs modifies the proliferation of leukemia MOLT-4 cells and induces their apoptosis through up-regulating Bax, caspase-3, and-8, and down-regulating Bcl-2 expression

Currently, it has been suggested that human mesenchymal stem cells (MSCs), a well-known multipotent cells, could modify the biological process in leukemia cells. Therefore, we evaluated MSCs’ possible effects on apoptosis and proliferation of acute lymphoblastic (ALL) MOLT-4 Accordingly, were co-cultured with MSCs. Then, 24, 48, 72 hours after treatment, percentages cell was estimated using flow cytometry analysis. Also, rates measured by MTT assay treatment. Finally, expression ratio candidate genes, including Bax, Bcl-2, caspase-3, -8, Based results, MSCs stimulated 48 but not 24 treatment compared control group (MOLT-4 cells). induced robust reduction rate group. On other hand, Real-Time PCR results indicated decrease Bcl-2 expression, conversely promotion -8 at mRNA levels. In sum, signified exert anti-leukemic through promoting Bax/Bcl-2 ration concomitant up-regulating caspases expression.